Surprising Arrangement of DNA in the Cell’s Nucleus Revealed by Novel Imaging Method

Left: A 3D illustration of the nucleus representing the classical theory of DNA organization at its center. Right: Turning the ramen bowl on its head – Microscopic image of the nucleus of a fruit fly larva’s muscle cell. The long chains of DNA (red) are attached to the nuclear lamina (green) – the inner layer of the nuclear membrane. Credit: Weizmann Institute of Science

On the Outskirts of the Nucleus

If you open a biology textbook and run through the images depicting how DNA is organized in the cell’s nucleus, chances are you’ll start feeling hungry; the chains of DNA would seem like a bowl of ramen: long strings floating in liquid. However, according to two new studies – one experimental[1] and the other theoretical[2] – that are the outcome of the collaboration between the groups of Prof. Talila Volk of the Molecular Genetics Department and Prof. Sam Safran of the Chemical and Biological Physics Department at the Weizmann Institute of Science, this image should be reconsidered. Clarifying it is essential since DNA’s spatial arrangement in the nucleus can affect the expression of genes contained within the DNA molecule, and hence the proteins found in the cell.

This story began when Volk was studying how mechanical forces influence cell nuclei in the muscle and found evidence that muscle contractions had an immediate effect on gene expression patterns. “We couldn’t explore this further because existing methods relied on imaging of chemically preserved cells, so they failed to capture what happens in the cell nuclei of an actual working muscle,” she says.

DNA Cell Nucleus Researchers

(Left to right) Prof. Talila Volk, Prof. Sam Safran, Dr. Dana Lorber, Dr. Daria Amiad-Pavlov and Dr. Adriana Reuveny. Moving away from the center. Credit: Weizmann Institute of Science

To address this issue, Dr. Dana Lorber, a research associate in Volk’s group, led the design of a device that makes it possible to study muscle nuclei in live fruit fly larvae. The device holds the tiny, translucent larva within a groove that allows it to contract and relax its muscles but keeps its movement constrained so that it can be scanned by a fluorescence microscope. Using the device, the researchers obtained images of the internal, linearly-organized complexes of DNA and its proteins (known as chromatin), surrounded by the membrane of the muscle nuclei.

Expecting a bowl full of ramen, Lorber and Dr. Daria Amiad-Pavlov, a postdoctoral fellow in Volk’s group, were in for a surprise. Rather than filling up the entire volume of the nucleus, the “noodles,” or long chromatin molecules, were organized as a relatively thin layer, attached to its inner walls. Similar to the outcome of the interaction between oil and water, what is known as “phase separation,” the chromatin separated itself from the bulk of the liquid inside of the nucleus and found its place at its outskirts, while most of the fluid medium remained at the center. The researchers realized that they were on their way to addressing a fundamental biological question, that is – how is chromatin, and hence DNA, organized in the nucleus in a living organism. “But the findings were so unexpected, we had to make sure no error had crept in and that this organization was universal,” Lorber says.

The surprising findings address a fundamental biological question – how is DNA organized in the nucleus in a living organism.

After teaming up with Safran’s group, they came to the conclusion there’d been no mistake. Safran and postdoctoral fellow Dr. Gaurav Bajpai built a theoretical model that included the physical factors governing chromatin organization in the nucleus, such as the relative forces of attraction between chromatin and its liquid environment and between chromatin and the nuclear membrane. The model predicted that the chromatin should undergo separation from the liquid phase, depending on the relative amount of liquid (hydration) in the nucleus. Furthermore, the phase separated chromatin could then arrange itself along the inside of the nuclear membrane – just as Volk’s team had found in their experiments.

Gaurav Bajpai

Dr. Gaurav Bajpai. Credit: Weizmann Institute of Science

The groups also explained why in previous studies by other scientists, the chromatin appeared to fill the cell nuclei. “When scientists plate cells on a glass slide in order to study them under a microscope, they change their volume and physically flatten them. This may perturb some of the forces governing chromatin arrangement and reduce the distance between the upper part of the nucleus to its base,” Safran explains.

To make sure these findings were not limited to fruit fly muscle cells, Lorber and Amiad-Pavlov joined forces with Dr. Francesco Roncato from Prof. Ronen Alon’s group of the Immunology Department and examined live human white blood cells. In this case too, the chromatin was similarly organized as a layer lining the inner nuclear wall. “This showed that what we’d found was likely to be a general phenomenon, and that this chromatin organization had probably been conserved throughout evolution,” says Amiad-Pavlov.

3D Chromatin Simulations

3D chromatin simulations reveal that chromatin organization in the nucleus is dependent on the physical interaction between chromatin and the nuclear lamina. When these interactions weaken (left to right) – as is the case in several diseases ranging from muscle dystrophies to neurological disorders – the chromatin shifts from the periphery of the nucleus to its center. Credit: Weizmann Institute of Science

The study opens up new avenues of research into DNA’s organization in the cell and, by extension, into the physical forces that act upon the nucleus and chromatin that can affect gene expression. One potential direction is exploring whether there’s a difference between DNA organization in health and disease. If so, this difference may be exploited in diagnosis, for example, as a new parameter for detecting cancer cells. In the study of embryonic development, exploring DNA organization may help clarify whether mechanical forces affect the differentiation of cells into new fates. Finally, it’s known that stiffness of the surface on which cells are placed can alter the expression of their genes. The new study suggests this may have to do with the surface’s push and pull on the nuclear membrane and the resultant impact on DNA organization within the nucleus. A better understanding of this interplay may help control gene expression in cells employed for engineering tissues with desired properties.

DNA and its chromatin packaging was thought to fill up to 60% of the nuclear volume. In their study, Weizmann Institute scientists found it to be 31%.


“Live imaging of chromatin distribution reveals novel principles of nuclear architecture and chromatin compartmentalization” by Daria Amiad-Pavlov, Dana Lorber, Gaurav Bajpai, Adriana Reuveny, Francesco Roncato, Ronen Alon, Samuel Safran and Talila Volk, 2 June 2021, Science Advances.
DOI: 10.1126/sciadv.abf6251

“Mesoscale phase separation of chromatin in the nucleus” by Gaurav Bajpai, Daria Amiad Pavlov, Dana Lorber, Talila Volk and Samuel Safran, 4 May 2021, eLife.
DOI: 10.7554/eLife.63976

Also taking part in the experimental study was Dr. Adriana Reuveny from Prof. Talila Volk’s group in the Molecular Genetics Department.

Prof. Talila Volk is the incumbent of the Sir Ernst B. Chain Professorial Chair.

Prof. Volk’s research is supported by the Aharon Katzir-Katchalsky Center; the Benoziyo Endowment Fund for the Advancement of Science; and the Henry Chanoch Krenter Institute for Biomedical Imaging and Genomics.

Prof. Samuel Safran is the incumbent of the Fern & Manfred Steinfeld Professorial Chair.

Prof. Safran’s research is supported by the Henry Chanoch Krenter Institute for Biomedical Imaging and Genomics; and the Harold Perlman Family

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